Microwave fixation of labile metabolites

proceedings of an official Satellite Symposium of the 8th International Congress of Pharmacology held in Tokyo, Japan, on 25 July 1981

Publisher: Pergamon in Oxford

Written in English
Cover of: Microwave fixation of labile metabolites |
Published: Pages: 191 Downloads: 546
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Subjects:

  • Neuropharmacology.,
  • Central nervous system -- Effect of drugs on.

Edition Notes

Includes bibliographies and index.

Statementeditors C. LeRoy Blank, William B. Stavinoha, Yuji Maruyama.
SeriesAdvances in the biosciences -- v.45, Advances in the biosciences -- v.45.
ContributionsBlank, C. LeRoy., Stavinoha, William B., Maruyama, Yuji., International Congress of Pharmacology, (8th : 1981 : Nagasaki)
Classifications
LC ClassificationsRM315
The Physical Object
Paginationxii,191p. :
Number of Pages191
ID Numbers
Open LibraryOL20967535M
ISBN 10008029829X

Microwave assistance allowed analyses to be approximately to orders of magnitude faster than the conventional immersion fixation method. Incorporation of microwave-mediated fixation in conjunction with microscale sample deposition onto matrix-assisted laser desorption ionization (MALDI) target plates has allowed the detection of. brain metabolism pp , In Mark. Ns an Rodnighd Rt (eds): Research Method isn Neurochemistry, Vol 2, New York, Plenum Press, Medina MA, Jone DJ, Stavinohs a WB, Ross DH: The levels of labile in-termediary metabolite in mouss e brain following rapid tissue fixation with microwave irradiation J Neuroche. 24m: , Jump to Content Jump to Main Navigation. Sign in Sign up Home COVID Journals Publish Before Print For Authors For Librarians About Us Submit Subscribe. I prefer cold extraction method by percolation, simply because some of the bioactive secondary metabolites present in the plant are heat labile and can be easily denatured in case you are.

Microwave irradiation. A specific type of fixation by heating, generated in this case by non-ionizing radiations at a frequency of GHz. The irradiation can occur on a fresh tissue sitting in an isotonic solution (primary microwave stabilization) or, in alternative, the specimen can be irradiated while immersed in a fixing agent, such as.

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Microwave Fixation of Labile Metabolites documents the proceedings of an official Satellite Symposium of the 8th International Congress of Pharmacology held in Tokyo, Japan, on 25 July The Symposium was entitled ""Drug Effects on Rapidly Metabolized Compounds in the CNS: Rapid Tissue Fixation with Microwave Irradiation."".

Purchase Microwave Fixation of Labile Metabolites - 1st Edition. Print Book & E-Book. ISBN  Microwave Fixation of Labile Metabolites 1st Edition by Yosh Maruyama (Editor), C.

Blank (Editor), W. Stavinoha (Editor) & ISBN ISBN X. Why is ISBN important. ISBN. This bar-code number lets you verify that you're getting exactly the right version or edition of a book.

The digit and digit formats Price: $   Microwave Fixation of Labile Metabolites documents the proceedings of an official Satellite Symposium of the 8th International Congress of Pharmacology held in Tokyo, Japan, on 25 July The Symposium was entitled """"Drug Effects on Rapidly Metabolized Compounds in the CNS: Rapid Tissue Fixation with Microwave Irradiation."""".

Book contents; Microwave Fixation of Labile Metabolites. Microwave Fixation of Labile Metabolites. Proceedings of an Official Satellite Symposium of the 8th International Congress of Pharmacology Held in Tokyo, Japan, on 25 July Cited by: 1.

Book contents; Microwave Fixation of Labile Metabolites. Microwave Fixation of Labile Metabolites. Proceedings of an Official Satellite Symposium of the 8th International Congress of Pharmacology Held in Tokyo, Japan, on 25 JulyPages The fast microwave fixation method, using a commercially available device, was successfully used to preserve granule-bound rat mast cell chymase which was subsequently detected by a postembedding immunogold procedure.

InMicrowave Fixation of Labile Metabolites, Vol. 45,Advances in Bioscience (edited by Blank, C. L., Stavinoha, W. Author Elsevier Books Reference. tmptmp. Author Frontiers. Microwave Fixation of Labile Metabolites: Proceedings of an Official Satellite Symposium of the 8th International Congress of Pharmacology Held in Tokyo, Japan, on 25 July Author Elsevier Books.

Acute ischemia produces dynamic changes in labile metabolites. To capture snapshots of such acute metabolic changes, we utilized focused microwave. The levels of labile intermediary metabolites in mouse brain following rapid tissue fixation with microwave irradiation.

Medina MA, Jones DJ, Stavinoha WB, Ross DH. J Neurochem, 24(2), 01 Feb Cited by 55 articles | PMID: Medma M A., Jones D. J., Stavinoha W. B, and Ross R H. () The levels of labile intermediary metabolites in mouse brain following rapid tissue fixation with microwave irradiation J.

Neurochem. 24, – CrossRef Google Scholar. Get this from a library. Microwave fixation of labile metabolites: proceedings of an official satellite symposium of the 8th International Congress of Pharmacology held in Tokyo, Japan, on J [C LeRoy Blank; William B Stavinoha; Yūji Maruyama;].

Microwave Fixation Of Labile Metabolites Proceedings Of An Official Satellite Symposium Of The 8th International Congress Of kid guide to florida free book, polaris trail blazer parts manual, christs object lessons english edition, tige service manual, the jesuits and the artshotronix heat transfer press repair manual.

labile metabolites in heart It is useful in the fixation of energy metabolites that would otherwise fluctuate widely following Focused microwave irradiation is. Quantification of amounts and 13 C content of metabolites in brain tissue using high‐ resolution magic angle spinning 13 C NMR Established extraction procedures of brain tissue are time consuming and may result in degradation of labile substances.

and 15 min later the animals were subjected to microwave fixation of the brain. Search Results. Books 11 results.

Documents results. The levels of labile intermediary metabolites in mouse brain following rapid tissue fixation with microwave irradiation. Neurochem. 24, – () Google Scholar. Schmidt MJ, Schmidt DE, Robison GA. Cyclic adenosine monophosphate in brain areas: microwave irradiation as a means of tissue fixation.

Science. Sep 17; ()– Medina MA, Jones DJ, Stavinoha WB, Ross DH. The levels of labile intermediary metabolites in mouse brain following rapid tissue fixation with microwave. Tissue fixation procedures may be useful, Microwave stabilization “fixes” the tissue with minimal alteration in protein structural matrix allowing for almost no protein loss as compared to 40% loss with formalin fixation.

This could possibly allow for increased sensitivity of monoclonal staining of low concentration labile proteins. Acute ischemia produces dynamic changes in labile metabolites. To capture snapshots of such acute metabolic changes, we utilized focused microwave treatment to fix metabolic flow in vivo in hearts of mice 10 min after ligation of the left anterior descending artery.

The left ventricle was subdivided into short-axis serial slices and the metabolites were analyzed by capillary. After rapid inactivation of the enzymes responsible for glucose metabolism by microwave irradiation, concentrations of glucose in 20 regions of the mouse brain were estimated with combined gas chromatography-mass spectrometry (GC-MS).

The highest concentrations of glucose were found in the periventricular nuclei of the hypothalamus and nucleus preopticus. Author(s): Blank,C LeRoy; Stavinoha,William B; Maruyama,Yūji; International Congress of Pharmacology,(8th: Tokyo, Japan) Title(s): Microwave fixation of labile metabolites: proceedings of an official satellite symposium of the 8th International Congress of Pharmacology held in Tokyo, Japan, on J / editors, C.

LeRoy Blank. Abstract. Since the introduction of microwave irradiation for morphologic diagnosis and research just over 20 years ago, the applications of microwaves have expanded considerably beyond their initial use as a safe, clean and rapid method of tissue fixation.

Microwave assisted extraction 15 Accelerated solvent extraction 16 metabolites. The primary metabolites are sugar, amino acids, nucleic acid, etc.

Whereas decomposition of thermo-labile component and more solvent consuming method [12]. Sample preparation is one of the most important steps in metabolome analysis. The challenges of determining microbial metabolome have been well discussed within the research community and many improvements have already been achieved in last decade.

The analysis of intracellular metabolites is particularly challenging. Environmental perturbations may considerably affect microbial metabolism. A simple method for selective phosphorylation of ribityl containing starting materials to afford FMN derivatives is presented.

The method is in particular valuable for the synthesis of labile. Alkaloids Sparteine, a lupine alkaloid, was extracted from Lupinus mutabilis, with methanol: acetic acid (, v/v) in a common microwave oven and the microwave irradiation program used one to five cycles of 30 s with a cooling step in between and conduct to 20% more sparteine than was obtained with a shaken-flask extraction using the same.

Mice were anesthetized by inhalation of 1–2 % isoflurane in oxygen and aligned in water-jacketed animal holder for microwave irradiation in a Muromachi Microwave Fixation System (10 kW model). For phantom testing irradiation times from to ms were varied at 50 ms intervals at constant kW for each buffer solution tested in the water.

Abstract— The levels of several labile glycolytic and organic phosphate metabolites in mouse brain were determined following rapid inactivation with MHz microwave irradiation. The levels of ATP in mouse brain following a 025 s exposure in a 6 kW microwave oven was found to be 2 ± 0 Whole brain levels of 8 labile intermediary metabolites in 04 s irradiated.

This paper describes a new method for the rapid fixation of labile lipid pools in the brain. Perfusion of the brain with % saline containing esterase inhibitors (p-bromphenacyl-bromide and diisopropyl fluorophosphate), an antioxidant (nordihydroguaiaretic acid) and a Ca2+ chelator (EDTA) resulted in a substantial reduction in the levels of.

A nonclassical heating technique using microwaves, termed “Bunsen burner of the 21st century”, is rapidly becoming popular and is dramatically reducing reaction times.

The significant outcomes of microwave (MW)-assisted green chemistry endeavors, which have resulted in the development of synthetic protocols for drugs and fine chemicals synthesis that are relatively .Biological nitrogen fixation (BNF) occurs when atmospheric nitrogen is converted to ammonia by an enzyme called nitrogenase.

The reaction for BNF is: \[\ce{N2 + 8 H^{+} + 8 e^{−} → 2 NH3 + H2}.\] This type of reaction results in N 2 gaining electrons (see above equation) and is thus termed a reduction reaction. The exact mechanism of. Cyanobacteria develop specialised nitrogen-fixing cells in order to solve their problem of managing theco-existence of oxygen labile nitrogen fixation andoxygen producing photosynthesis (FayGallon).

Heterocysts provide the ability to perform aerobic nitrogen fixation through the exclusion of atmosphericoxygen and oxygenic photosynthesis.